Role of neutrophil-mediated inflammation in aspirin-induced gastric mucosal injury

Dig Dis Sci. 1995 Nov;40(11):2300-4. doi: 10.1007/BF02063228.

Abstract

The objectives of this study were to determine the roles of neutrophil-endothelial cell interactions and oxygen-derived free radicals in the pathogenesis of aspirin-induced gastric mucosal injury in rats. Oral administration of acidified aspirin (200 mg/kg) resulted in linear hemorrhagic erosions and an increase in myeloperoxidase activity, an index of neutrophil infiltration, in the gastric mucosa. Aspirin-induced gastric damage and the increase in myeloperoxidase activity were significantly inhibited by the injection of anti-CD11a, anti-CD11b, anti-intercellular adhesion molecule-1 monoclonal antibodies, and the combination of superoxide dismutase and catalase, which are scavengers of active oxygen species. These results suggest that neutrophil-endothelial adhesive interactions, which occur via CD11a/ CD18- and CD11b/CD18-dependent interactions with intercellular adhesion molecule-1, and oxygen-derived free radicals produced by neutrophils are implicated in the production of aspirin-induced gastric mucosal injury.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal / toxicity*
  • Antibodies, Monoclonal
  • Aspirin / toxicity*
  • CD11 Antigens / immunology
  • CD11 Antigens / physiology
  • Catalase / pharmacology
  • Free Radicals / metabolism
  • Gastric Mucosa / drug effects*
  • Gastric Mucosa / enzymology
  • Gastric Mucosa / pathology
  • Gastritis / chemically induced
  • Gastritis / pathology
  • Gastritis / physiopathology
  • Intercellular Adhesion Molecule-1 / immunology
  • Intercellular Adhesion Molecule-1 / physiology
  • Male
  • Neutrophils / physiology*
  • Peroxidase / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Superoxide Dismutase / pharmacology

Substances

  • Anti-Inflammatory Agents, Non-Steroidal
  • Antibodies, Monoclonal
  • CD11 Antigens
  • Free Radicals
  • Intercellular Adhesion Molecule-1
  • Catalase
  • Peroxidase
  • Superoxide Dismutase
  • Aspirin