Fission yeast Rep2 is a putative transcriptional activator subunit for the cell cycle 'start' function of Res2-Cdc10

EMBO J. 1995 Oct 2;14(19):4794-802.

Abstract

In the yeast cell cycle 'start' requires sets of the Cdc10/ SWI family of transcriptional factors which activate the MCB cis elements contained in genes essential for S phase progression. Fission yeast possess two such overlapping systems, Res1-Cdc10 and Res2-Cdc10, both of which act to start the mitotic and meiotic cycles. We have recently isolated rep2+ as a multicopy suppressor of a temperature-sensitive cdc10 mutant which encodes a zinc finger protein. Here we show that the Rep2 zinc finger protein is an essential component of the active Res2-Cdc10 transcriptional regulator complex and likely to play a role in the control of cell cycle 'start'. Our data suggest that Rep2 is a transcriptional activator subunit which interacts with the MCB binding subunit complex formed by Res2 and Cdc10.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Cycle Proteins / metabolism*
  • Cell Cycle Proteins / physiology
  • Cloning, Molecular
  • DNA / metabolism
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • DNA-Binding Proteins / physiology*
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • Fungal Proteins / physiology
  • G1 Phase / genetics*
  • Genes, Fungal / genetics
  • Genes, Regulator / genetics
  • Meiosis / genetics
  • Mitosis / genetics
  • Models, Genetic
  • Molecular Sequence Data
  • Nitrogen / physiology
  • Schizosaccharomyces / genetics
  • Schizosaccharomyces / growth & development
  • Schizosaccharomyces pombe Proteins*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Suppression, Genetic
  • Trans-Activators / chemistry
  • Trans-Activators / genetics
  • Trans-Activators / metabolism
  • Trans-Activators / physiology*
  • Transcription Factors
  • Zinc Fingers / genetics

Substances

  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • Fungal Proteins
  • Rep2 protein, S pombe
  • Schizosaccharomyces pombe Proteins
  • Trans-Activators
  • Transcription Factors
  • cdc10 protein, S pombe
  • rep1 protein, S pombe
  • res2 protein, S pombe
  • DNA
  • Nitrogen

Associated data

  • GENBANK/X91044