Effect of sulfated xylans during the interaction of [125I]-thrombin with antithrombin III or heparin cofactor II of human plasma

Eur J Drug Metab Pharmacokinet. 1995 Jan-Mar;20(1):73-7. doi: 10.1007/BF03192291.

Abstract

[125I]-Labeled thrombin was incubated with human plasma and its interactions with the two plasma protease inhibitors antithrombin III (AT-III) or heparin cofactor II (HC-II) were investigated in the presence of oat spelts xylan sulfate (OSXS), sodium pentosan polysulfate (SP-54), and the results were compared with heparin and dermatan sulfate. Addition of OSXS or SP-54 enhanced the complexation of thrombin with HC-II or with both AT-III and HC-II depending upon the concentration and the duration of the interactions of the sulfated compounds with plasma. During the 30 s interaction, OSXS and SP-54 enhanced both AT-III-thrombin and HC-II-thrombin interaction while heparin was more selective and enhanced only the AT-III-thrombin interaction. However after a 2 min interaction, heparin showed an increase in the HC-II-thrombin interaction at higher concentrations. The complexations of AT-III-thrombin and HC-II-thrombin were reversed in the presence of 200 micrograms/ml of SP-54 during a 30 s interaction or in presence of 100 micrograms/ml of OSXS during a 2 min interaction. The Western blotting method of detecting thrombin showed that during the 10 s interaction, heparin enhanced the thrombin-AT-III complex formation while OSXS enhanced the thrombin-HC-II complex formation.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antithrombin III / metabolism*
  • Blotting, Western
  • Dermatan Sulfate / pharmacology
  • Electrophoresis, Polyacrylamide Gel
  • Heparin / pharmacology
  • Heparin Cofactor II / metabolism*
  • Humans
  • Iodine Radioisotopes
  • Pentosan Sulfuric Polyester / pharmacology
  • Sodium Dodecyl Sulfate
  • Sulfates / pharmacology
  • Thrombin / metabolism*
  • Xylans / pharmacology*

Substances

  • Iodine Radioisotopes
  • Sulfates
  • Xylans
  • Dermatan Sulfate
  • Sodium Dodecyl Sulfate
  • Pentosan Sulfuric Polyester
  • Heparin Cofactor II
  • Antithrombin III
  • Heparin
  • Thrombin