CD27/CD70 interaction directly drives B cell IgG and IgM synthesis

Eur J Immunol. 1995 Oct;25(10):2825-9. doi: 10.1002/eji.1830251017.


CD27 is a T cell activation antigen expressed on a majority of peripheral blood T cells. CD27 is also expressed on a subpopulation of human B cells, and it is reported that CD27+ B cells secrete both IgG and IgM. CD70, a ligand for CD27, is expressed on activated T and B cells, suggesting an interaction between T and B cells via CD27/CD70 ligation. Here, we analyze B cell immunoglobulin synthesis using a CD70 transfectant and present functional data showing that B cells secrete large amounts of IgG and IgM as a result of the CD27/CD70 interaction. A flow cytometric analysis showed that CD27 expression was increased and CD70 was expressed on tonsillar and peripheral blood B cells after activation with Staphylococcus aureus Cowan strain (SAC) plus interleukin (IL-2). In addition, the proliferation of B cells was enhanced mildly by the addition of CD70 transfectant, and its proliferation was blocked by anti-CD70 mAb. More importantly, the CD70 transfectant enhanced IgG and IgM production by purified B cells greatly in the presence of SAC plus IL-2. The enhancement was completely blocked by the addition of either anti-CD70 mAb or anti-CD27 mAb. Strongly suggesting that the interaction of CD27 with its ligand, CD70, on B cells plays an important role in B cell growth and differentiation to produce IgG and IgM.

MeSH terms

  • Adult
  • Antibodies, Monoclonal / pharmacology
  • Antigens, CD*
  • B-Lymphocytes / drug effects
  • B-Lymphocytes / immunology*
  • Blood Cells
  • CD27 Ligand
  • Flow Cytometry
  • Humans
  • Immunoglobulin G / biosynthesis*
  • Immunoglobulin M / biosynthesis*
  • Interleukin-2 / pharmacology
  • Ligands
  • Lymphocyte Activation* / drug effects
  • Lymphocyte Cooperation
  • Membrane Proteins / genetics
  • Membrane Proteins / immunology*
  • Palatine Tonsil / cytology
  • Recombinant Proteins / metabolism
  • Staphylococcus aureus / immunology
  • Transfection
  • Tumor Necrosis Factor Receptor Superfamily, Member 7 / immunology*


  • Antibodies, Monoclonal
  • Antigens, CD
  • CD27 Ligand
  • CD70 protein, human
  • Immunoglobulin G
  • Immunoglobulin M
  • Interleukin-2
  • Ligands
  • Membrane Proteins
  • Recombinant Proteins
  • Tumor Necrosis Factor Receptor Superfamily, Member 7