Differential effects of interleukin-10 on the expression of HLA class II and CD1 molecules induced by granulocyte/macrophage colony-stimulating factor/interleukin-4

Eur J Immunol. 1995 Sep;25(9):2465-70. doi: 10.1002/eji.1830250909.


Interleukin (IL)-10 down-regulates HLA class II molecules, whether constitutively expressed or up-regulated by interferon-gamma or IL-4 on monocytes but not on B lymphocytes. In this study we show that IL-10 does not inhibit HLA class II expression induced by the combination granulocyte/macrophage colony-stimulating factor and IL-4 on monocytes, although it simultaneously abrogates the expression of CD1 molecules induced by the same combination of cytokines. CD1 molecules can act as element of genetic restriction for CD4- CD8- T lymphocytes, and the suppression of CD1 expression by IL-10 abolished antigen presentation to CD1-restricted CD4- CD8- T cell receptor-positive T cells. Although HLA class II expression was not down-regulated by IL-10, the antigen specific proliferative response of CD4+ T cells was nevertheless decreased. This was not caused by down-regulation of known co-stimulatory molecules such as B7.1, B7.2 and ICAM-1. IL-10 decreased the antigen specific proliferative response further by directly influencing the T lymphocytes. Our results indicate that IL-10 exerts some of its immunoregulatory functions by differential modulation of antigen presenting molecules, induced by the same combination of cytokines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigen Presentation
  • Antigens, CD1 / biosynthesis*
  • B7-1 Antigen / metabolism
  • Cell Division / drug effects
  • Cell Division / immunology
  • Cells, Cultured
  • Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology*
  • Histocompatibility Antigens Class II / biosynthesis*
  • Histocompatibility Antigens Class II / immunology
  • Humans
  • Interleukin-10 / pharmacology*
  • Interleukin-4 / pharmacology*
  • Lymphocyte Activation / drug effects
  • Lymphocyte Activation / immunology
  • Monocytes / immunology*
  • Monocytes / metabolism
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / metabolism


  • Antigens, CD1
  • B7-1 Antigen
  • Histocompatibility Antigens Class II
  • Interleukin-10
  • Interleukin-4
  • Granulocyte-Macrophage Colony-Stimulating Factor