Expression of cytochrome P450 CYP1B1 in breast cancer

FEBS Lett. 1995 Oct 30;374(2):270-2. doi: 10.1016/0014-5793(95)01126-y.

Abstract

The expression of CYP1B1 has been identified in breast cancer using the reverse transcriptase-polymerase chain reaction and immunoblotting. CYP1B1 mRNA was expressed in the majority of breast tumours and immunoblotting of breast tumours identified a single protein band of molecular weight 60 kDa corresponding to the predicted molecular weight of human CYP1B1. This is the first study to identify CYP1B1 expression in a tumour where it may represent a previously unknown pathway for the metabolism of oestradiol and chemotherapeutic drugs.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Aryl Hydrocarbon Hydroxylases*
  • Base Sequence
  • Breast Neoplasms / enzymology*
  • Breast Neoplasms / pathology
  • Cytochrome P-450 CYP1B1
  • Cytochrome P-450 Enzyme System / biosynthesis*
  • Cytochrome P-450 Enzyme System / genetics
  • DNA Primers
  • Female
  • Humans
  • Immunoblotting
  • Middle Aged
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA, Messenger / metabolism
  • Transcription, Genetic

Substances

  • DNA Primers
  • RNA, Messenger
  • Cytochrome P-450 Enzyme System
  • Aryl Hydrocarbon Hydroxylases
  • CYP1B1 protein, human
  • Cytochrome P-450 CYP1B1