Helix-loop-helix transcription factors regulate Id2 gene promoter activity

FEBS Lett. 1995 Oct 30;374(2):279-83. doi: 10.1016/0014-5793(95)01128-2.

Abstract

Id-like helix-loop-helix (HLH) transcription factors are involved in the regulation of proliferation and differentiation of several cell types. We isolated 5' regulatory region of mouse Id2 gene and demonstrated that it contains several E-box clusters. These E-boxes mediate stimulatory effects of basic-HLH (bHLH) transcription factors ME1, ME2, and NSCL1 on Id2 promoter activity. Co-expression of Id2 blocks the stimulatory effect of bHLH transcription factors which suggests the presence of feedback loops in Id2 transcriptional regulation. Overexpression of NSCL1 in F9 cells blocks the downregulation of Id2 gene expression during retinoic acid induced differentiation. Our data demonstrate that bHLH transcription factors regulate Id2 gene expression.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • Basic Helix-Loop-Helix Transcription Factors
  • Cell Differentiation
  • DNA, Complementary
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism*
  • Down-Regulation
  • Gene Expression Regulation
  • Helix-Loop-Helix Motifs / physiology*
  • Inhibitor of Differentiation Protein 2
  • Mice
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • Promoter Regions, Genetic*
  • Repressor Proteins*
  • TCF Transcription Factors
  • Transcription Factor 4
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Tumor Cells, Cultured

Substances

  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • Basic Helix-Loop-Helix Transcription Factors
  • DNA, Complementary
  • DNA-Binding Proteins
  • Idb2 protein, mouse
  • Inhibitor of Differentiation Protein 2
  • Nerve Tissue Proteins
  • Nhlh1 protein, mouse
  • Repressor Proteins
  • TCF Transcription Factors
  • Tcf12 protein, mouse
  • Tcf4 protein, mouse
  • Transcription Factor 4
  • Transcription Factors
  • TCF12 protein, human