Human estrogen receptor messenger RNA variants in both normal and tumor breast tissues

Mol Cell Endocrinol. 1995 Jul;112(1):1-13. doi: 10.1016/0303-7207(95)03576-s.


By using the PCR-SSCP technique we characterized various ER-specific RNA species present in a series of primary breast cancers, as well as in cell lines established from breast carcinomas and in mammary gland tissues from healthy specimens. A series of six truncated messenger RNAs generated by alternative splicing was characterized. These RNAs correspond to specific deletions of one (exons 2-7, except exon 6) or two (exons 3 + 4) exons. All these RNA variants are observed in each one of the analyzed RNAs, regardless of origin. In addition, the relative amount of these different variants in ER + tumors is comparable to that measured in ER - tumors and healthy mammary gland tissues. This data suggests that tumor progression is not related to the emergence of any of the ER mRNA variants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing
  • Base Sequence
  • Breast / chemistry*
  • Breast Neoplasms / genetics*
  • DNA, Complementary
  • Electrophoresis, Agar Gel
  • Exons
  • Female
  • Gene Deletion*
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Polymorphism, Single-Stranded Conformational
  • RNA, Messenger / analysis*
  • RNA-Directed DNA Polymerase
  • Receptors, Estrogen / genetics*
  • Templates, Genetic
  • Tumor Cells, Cultured


  • DNA, Complementary
  • RNA, Messenger
  • Receptors, Estrogen
  • RNA-Directed DNA Polymerase