Nitrogen-source-induced activation of neutral trehalase in Schizosaccharomyces pombe and Pachysolen tannophilus: role of cAMP as second messenger

FEMS Microbiol Lett. 1995 Oct 15;132(3):229-32. doi: 10.1016/0378-1097(95)00269-b.

Abstract

Resting cells of the fission yeast Schizosaccharomyces pombe, suspended in buffer with glucose, responded to the addition of asparagine by increasing trehalase activity. This response was preceded by a peak in cAMP concentration. The addition of the nitrogen source to resting cells, devoid of the catalytic subunit of cAMP-dependent protein kinase, produced the transient increase in cAMP but did not promote any change in trehalase activity. In the budding yeast Pachysolen tannophilus, the activation of trehalase by nitrogen source was also accompanied by a sharp peak in cAMP. These results suggest that in the two yeasts cAMP acts as a second messenger in the transduction of the nitrogen-source-induced signal causing the activation of trehalase.

Publication types

  • Comparative Study

MeSH terms

  • Asparagine / pharmacology*
  • Cyclic AMP / metabolism*
  • Cyclic AMP-Dependent Protein Kinases / metabolism
  • Enzyme Activation
  • Saccharomycetales / drug effects
  • Saccharomycetales / enzymology*
  • Schizosaccharomyces / drug effects
  • Schizosaccharomyces / enzymology
  • Second Messenger Systems*
  • Trehalase / metabolism*

Substances

  • Asparagine
  • Cyclic AMP
  • Cyclic AMP-Dependent Protein Kinases
  • Trehalase