The Drosophila melanogaster homolog of the mammalian MAPK-activated protein kinase-2 (MAPKAPK-2) lacks a proline-rich N-terminus

Gene. 1995 Oct 3;163(2):209-14. doi: 10.1016/0378-1119(95)00279-f.


Recently, a mammalian kinase cascade was discovered that is triggered by stress and heat shock, and leads to the stimulation of mitogen-activated protein kinase (MAPK)-activated protein kinase-2 (MAPKAPK-2). Surprisingly, this process turns out to be independent of the classical MAPK. The stress-induced activation of MAPKAPK-2, in turn, results in the phosphorylation of small heat-shock proteins (Hsp). We have isolated a Drosophila melanogaster (Dm) cDNA encoding a polypeptide that has extensive sequence similarity to the mammalian MAPKAPK-2. As in mammalian MAPKAPK-2, the Dm MAPKAPK-2 possesses a MAPK phosphorylation site and a nuclear targeting sequence located C-terminal to the catalytic domain. However, in contrast to its mammalian counterpart, it lacks the Pro-rich N-terminal region proposed to form Src-homology domain 3 (SH3) binding domains. A 2.4-kb MAPKAPK-2 message is expressed throughout development, while two shorter transcripts of 2.3 and 1.8 kb appear to be specifically expressed in the germline. The 1.8-kb transcript results from the usage of an atypical germline-specific polyadenylation signal (AATATA) located early within the 3' untranslated region. Dm MAPKAPK-2 is located at cytological position 5D in the Dm genome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Cloning, Molecular
  • Drosophila melanogaster / genetics*
  • Heat-Shock Proteins / genetics*
  • MAP Kinase Kinase 2
  • Mitogen-Activated Protein Kinase Kinases*
  • Molecular Sequence Data
  • Proline / genetics
  • Protein-Serine-Threonine Kinases / chemistry
  • Protein-Serine-Threonine Kinases / genetics*
  • Protein-Tyrosine Kinases / chemistry
  • Protein-Tyrosine Kinases / genetics*
  • Sequence Alignment
  • Sequence Analysis


  • Heat-Shock Proteins
  • Proline
  • Protein-Tyrosine Kinases
  • Protein-Serine-Threonine Kinases
  • MAP Kinase Kinase 2
  • Mitogen-Activated Protein Kinase Kinases

Associated data

  • GENBANK/U20757