Rat intestinal crypt-cell replication factor with homology to early S-phase proteins required for cell division

Gene. 1995 Oct 3;163(2):243-7. doi: 10.1016/0378-1119(95)00297-j.

Abstract

Cell proliferation requires inhibitory and permissive factors to monitor cell-cycle progression and control DNA replication. The small intestine has a high rate of proliferation and a very low incidence of cancer, suggestive of efficient mechanisms for control of the cell cycle and assuring fidelity of DNA replication. We have isolated a cDNA from a rat crypt-cell library which hybridized to a 3.0-kb mRNA specific for crypt cells, the proliferative cell compartment of the intestine. Its amino-acid sequence indicates that it is a new member of a family of replication proteins found in yeast, Cenorhabditis elegans, mouse and humans. Its transcripts were markedly increased in fetal rat intestine and liver, decreased in long-term confluent and serum-starved tissue culture cells (IEC cells, a cell line derived from rat crypt cells), increased with serum repletion as cells resumed proliferation, and appeared to be species specific. Isolation and functional characterization of small intestinal crypt-cell replication factors should help explain this organ's low incidence of cancer.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Cycle Proteins / biosynthesis
  • Cell Cycle Proteins / genetics*
  • Cell Cycle Proteins / isolation & purification
  • Cell Division
  • Cell Line
  • Cloning, Molecular
  • Intestine, Small / cytology
  • Intestine, Small / metabolism*
  • Molecular Sequence Data
  • Rats
  • S Phase
  • Sequence Alignment

Substances

  • Cell Cycle Proteins

Associated data

  • GENBANK/U17565