Analysis of the DnaK molecular chaperone system of Francisella tularensis

Gene. 1995 Oct 16;164(1):149-52. doi: 10.1016/0378-1119(95)00489-s.


We have cloned the Francisella tularensis (Ft) grpE-dnaK-dnaJ heat-shock genes which are organized in that order. These genes allow heterologous genetic complementation of each respective mutant strain of Escherichia coli (Ec) for bacteriophage lambda growth. The nucleotide sequences of the Ft grpE-dnaK-dnaJ genes and the deduced amino-acid sequences share significant homologies with their respective Ec counterparts. The Ft DnaK and DnaJ proteins cross-react with polyclonal antibodies raised against the respective Ec proteins. The grpE-dnaK-dnaJ genes of Ft are organized in a fashion that is more characteristic of Gram+ bacteria.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins / biosynthesis
  • Bacterial Proteins / genetics*
  • Blotting, Western
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Escherichia coli Proteins*
  • Francisella tularensis / genetics*
  • HSP40 Heat-Shock Proteins
  • HSP70 Heat-Shock Proteins / biosynthesis
  • HSP70 Heat-Shock Proteins / genetics*
  • Heat-Shock Proteins / biosynthesis
  • Heat-Shock Proteins / genetics*
  • Molecular Chaperones / biosynthesis
  • Molecular Chaperones / genetics*
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Recombinant Proteins / biosynthesis
  • Sequence Analysis, DNA


  • Bacterial Proteins
  • DnaJ protein, E coli
  • Escherichia coli Proteins
  • GrpE protein, Bacteria
  • GrpE protein, E coli
  • HSP40 Heat-Shock Proteins
  • HSP70 Heat-Shock Proteins
  • Heat-Shock Proteins
  • Molecular Chaperones
  • Recombinant Proteins
  • dnaK protein, E coli

Associated data

  • GENBANK/K01298
  • GENBANK/L43367
  • GENBANK/M12565
  • GENBANK/M84964
  • GENBANK/X08763