pDblet, a stable autonomously replicating shuttle vector for Schizosaccharomyces pombe

Gene. 1995 Oct 16;164(1):173-7. doi: 10.1016/0378-1119(95)00497-t.


We have constructed a new multipurpose stable shuttle vector for the fission yeast Schizosaccharomyces pombe (Sp). Plasmid pDblet was designed to provide convenient features for molecular work and to overcome the inconveniences of previously designed Sp vectors. It contains the Sp ura4 gene as selectable marker and a new highly efficient ARS (autonomously replicating sequence) element, allowing the vector to remain stable as a monomer in Sp. In addition, pDblet transforms Sp with high efficiency and has high mitotic stability and low copy number.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • DNA Replication
  • Genetic Markers
  • Genetic Vectors*
  • Mitosis
  • Replication Origin
  • Schizosaccharomyces / genetics*
  • Selection, Genetic
  • Transformation, Genetic


  • Genetic Markers