The mitochondrial pyruvate dehydrogenase complex: nucleotide and deduced amino-acid sequences of a cDNA encoding the Arabidopsis thaliana E1 alpha-subunit

Gene. 1995 Oct 27;164(2):251-4. doi: 10.1016/0378-1119(95)00465-i.

Abstract

A cDNA encoding the E1 alpha subunit of the Arabidopsis thaliana (At) mitochondrial (mt) pyruvate dehydrogenase complex (PDC) was sequenced. The 1435-bp cDNA consists of a 1167-bp open reading frame encoding a 43.0-kDa polypeptide of 389 amino acids (aa) (pI 7.1). The plant E1 alpha subunit has 47-51% aa sequence identity with other eukaryotic sequences. Among the regions that are highly conserved are the aa surrounding phosphorylation sites 1 and 2 of the mammalian sequence, including the conserved Ser292 residue of At at site 1. An essential active site residue, Cys62 of the bovine subunit, is also conserved. A 32-aa presumptive mt targeting sequence is present at the N terminus.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Arabidopsis / enzymology*
  • Arabidopsis / genetics*
  • Base Sequence
  • Cattle
  • Conserved Sequence
  • Cysteine
  • Macromolecular Substances
  • Mitochondria / enzymology*
  • Molecular Sequence Data
  • Open Reading Frames
  • Pyruvate Dehydrogenase Complex / chemistry
  • Pyruvate Dehydrogenase Complex / genetics*
  • Sequence Homology, Amino Acid

Substances

  • Macromolecular Substances
  • Pyruvate Dehydrogenase Complex
  • Cysteine

Associated data

  • GENBANK/U21214
  • PIR/S20813
  • PIR/S23506