Determination of estrogen ring D glucuronides in pregnancy plasma by direct radioimmunoassay without hydrolysis

Clin Chim Acta. 1979 Jan 15;91(2):169-75. doi: 10.1016/0009-8981(79)90453-4.


A direct radioimmunoassay method using highly specific antisera without prior deconjugation has been developed for determination of estradiol 17-glucuronide and estriol 16-glucuronide in human plasma. Antisera were elicited in the rabbit by immunization with antigens in which the steroid haptens are linked to a carrier protein through the C-2 or C-4 position. After treatment with Rivanol the protein of albumin-free antiserum was covalently bound to a p-arylamine glass bead support through the cross-linkage with glutaraldehyde. A simple and reliable assay method employing the antibody-glass preparation was established and applied to measurement of estrogen ring D glucuronide concentration in peripheral plasma throughout normal pregnancy.

MeSH terms

  • Animals
  • Estradiol / blood*
  • Estriol / blood*
  • Female
  • Glucuronates / blood*
  • Humans
  • Immune Sera
  • Pregnancy*
  • Rabbits
  • Radioimmunoassay


  • Glucuronates
  • Immune Sera
  • Estradiol
  • Estriol