MDM2 gene amplification in bone and soft-tissue tumors: association with tumor progression in differentiated adipose-tissue tumors

Int J Cancer. 1995 Oct 20;64(5):342-6. doi: 10.1002/ijc.2910640511.


We have studied 107 bone and soft-tissue sarcomas and 8 lipomas for amplification of the MDM2 gene. This gene was amplified in 3 out of 67 osteosarcomas, 3 out of 20 malignant fibrous histiocytomas, 4 out of 20 liposarcomas, and 4 out of 8 lipomas. The amplification was associated with overexpression of mRNA. In osteosarcomas, contrary to previous findings, all amplifications were observed in primary lesions. In liposarcomas, the amplification was seen exclusively in well-differentiated tumors with high frequency (4/5) but not in other subtypes (0/15). In addition, MDM2 amplification was also frequently found in deep-seated intra- or intermuscular lipomas (4/5). Hence, it is suggested that MDM2 amplification plays a significant role in the development of differentiated adipose-tissue tumors. Three well-differentiated liposarcomas with MDM2 amplification coexisted with high-grade dedifferentiated sarcomas, in which MDM2 amplification was also observed. Interestingly, in 2 of these cases, the grades of amplification correlated with the histological grades, indicating an important role of MDM2 overexpression in tumor progression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Blotting, Northern
  • Bone Neoplasms / genetics*
  • Cell Differentiation / physiology
  • Disease Progression
  • Female
  • Gene Amplification*
  • Genes, p53
  • Histiocytoma, Benign Fibrous / genetics
  • Humans
  • Lipoma / genetics
  • Liposarcoma / genetics*
  • Male
  • Middle Aged
  • Mutation
  • Nuclear Proteins*
  • Osteosarcoma / genetics
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins c-mdm2
  • RNA, Messenger / analysis
  • Sarcoma / genetics
  • Soft Tissue Neoplasms / genetics*


  • Nuclear Proteins
  • Proto-Oncogene Proteins
  • RNA, Messenger
  • MDM2 protein, human
  • Proto-Oncogene Proteins c-mdm2