Identification of Enterohaemorrhagic Escherichia Coli by Means of Their Production of Enterohaemolysin

J Appl Bacteriol. 1995 Aug;79(2):178-80. doi: 10.1111/j.1365-2672.1995.tb00932.x.


With increasing interest in verocytotoxigenic Escherichia coli (VTEC) being associated with both human and animal infection, a simple system of rapidly identifying the majority of VTEC has been devised. This depends on the fact that most VTEC produce enterohaemolysin, which is rarely produced by non-VTEC. By employing two media, traditional sheep blood agar (SBA) and washed sheep blood agar supplemented with calcium (WSBA-Ca), enterohaemolysin-producing strains can be easily differentiated from other E. coli enabling most VTEC types, including ones belonging to serogroups other than O157, to be isolated.

MeSH terms

  • Animals
  • Bacterial Proteins / biosynthesis
  • Bacterial Toxins / biosynthesis*
  • Calcium / pharmacology*
  • Culture Media
  • Escherichia coli / genetics
  • Escherichia coli / isolation & purification*
  • Escherichia coli / metabolism
  • Escherichia coli Proteins*
  • Hemolysin Proteins / biosynthesis*
  • Humans
  • Sheep / blood
  • Shiga Toxin 1
  • Toxins, Biological


  • Bacterial Proteins
  • Bacterial Toxins
  • Culture Media
  • Escherichia coli Proteins
  • Hemolysin Proteins
  • Hlya protein, E coli
  • Shiga Toxin 1
  • Toxins, Biological
  • enterohemolysin
  • Calcium