Colocalization of vascular endothelial growth factor (vascular permeability factor) and insulin in pancreatic islet cells

J Clin Endocrinol Metab. 1995 Nov;80(11):3196-200. doi: 10.1210/jcem.80.11.7593426.


Previously, we found that the islet of pancreas stained with a antibody against the vascular permeability factor (VPF; also known as vascular endothelial growth factor, VEGF) protein. To determine how common this reaction was and whether it was a specific reaction for the islet, we examined its expression and specific cellular localization. Two different antibodies directed against VPF/VEGF peptide revealed an intense reaction for beta-cells in the human islets of Langerhans, and several human beta-cell tumors (insulinomas), but no reaction, were detectable in the vascular endothelium. In the fetal pancreas (second and third trimesters), the VPF/VEGF peptide was detected in immature islets. Northern blot analysis of cell lines derived from rodent insulinomas revealed expression of VPF/VEGF messenger ribonucleic acid. Western blot analysis of conditioned medium from one of these cell lines showed the presence of the released VPF/VEGF protein. These findings indicate that beta-cells have a specific role other than endocrine function in the pancreas. VPF/VEGF in beta-cells may be involved in the maintenance and control of permeability within the islet capillary system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Base Sequence
  • Blotting, Northern
  • Endothelial Growth Factors / metabolism*
  • Female
  • Humans
  • Immunoblotting
  • Immunohistochemistry
  • Insulin / metabolism*
  • Islets of Langerhans / metabolism*
  • Lymphokines / metabolism*
  • Male
  • Middle Aged
  • Molecular Probes / genetics
  • Molecular Sequence Data
  • Tissue Distribution
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors


  • Endothelial Growth Factors
  • Insulin
  • Lymphokines
  • Molecular Probes
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors