Pathogen antigen- and superantigen-reactive synovial fluid T cells in reactive arthritis

J Infect Dis. 1995 Nov;172(5):1290-7. doi: 10.1093/infdis/172.5.1290.

Abstract

Analysis of pathogen-reactive T cell clones (CD3+4+8-TCR alpha beta +), isolated from the synovial fluid of 2 HLA-B27-positive patients with Yersinia enterocolitica-triggered reactive arthritis, has provided important information about the cellular immune response to this disease-inciting pathogen. This study demonstrates that the proteins secreted by Y. enterocolitica, including a protein with tyrosine phosphatase activity (YopH), are potent immunogens stimulating CD4+ cells within the inflamed joint. The pathogen-reactive T cell clones preferentially utilized a limited set of T cell receptor variable region gene segments. A purified Yersinia superantigen triggered a proliferative response in most of the antigen-reactive T cell clones tested. These results suggest that the activity of this pathogen's superantigen influences the cellular immune response to its antigens.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Antigens, Bacterial / immunology*
  • Arthritis, Infectious / immunology*
  • Base Sequence
  • CD4-Positive T-Lymphocytes / immunology
  • Clone Cells
  • DNA Primers
  • DNA, Bacterial / genetics
  • Genetic Variation
  • HLA Antigens / analysis
  • Histocompatibility Testing
  • Humans
  • Immunity, Cellular
  • Immunophenotyping
  • Inflammation
  • Lymphocyte Activation
  • Male
  • Molecular Sequence Data
  • Phosphoprotein Phosphatases / immunology
  • Plasmids
  • Polymerase Chain Reaction
  • Protein Tyrosine Phosphatases / immunology
  • Receptors, Antigen, T-Cell, alpha-beta / genetics
  • Superantigens / immunology*
  • Synovial Fluid / immunology*
  • T-Lymphocytes / immunology*
  • Virulence / genetics
  • Yersinia Infections / immunology*
  • Yersinia enterocolitica / genetics
  • Yersinia enterocolitica / immunology*
  • Yersinia enterocolitica / isolation & purification

Substances

  • Antigens, Bacterial
  • DNA Primers
  • DNA, Bacterial
  • HLA Antigens
  • Receptors, Antigen, T-Cell, alpha-beta
  • Superantigens
  • Phosphoprotein Phosphatases
  • Protein Tyrosine Phosphatases