Comparison of the genomes of the wild-type French viscerotropic strain of yellow fever virus with its vaccine derivative French neurotropic vaccine

J Gen Virol. 1995 Nov;76 ( Pt 11):2749-55. doi: 10.1099/0022-1317-76-11-2749.

Abstract

The French neurotropic vaccine, or FNV, was used extensively in Africa to control yellow fever (YF). Although efficacious, the vaccine caused an unacceptable rate of post-vaccinal complications in children and was subsequently replaced by the 17D vaccine. Here we report that the genomes of the wild-type YF virus French viscerotropic virus and its attenuated vaccine derivative, FNV virus from the Institut Pasteur, Paris, (FNV-IP) differ by 77 nucleotides encoding 35 amino acid substitutions. Comparison of FNV-IP and three other isolates of FNV with other YF vaccine strains (17D-204 and 17DD derived from wild-type strain Asibi) revealed that during the two attenuation processes two common nucleotide changes arose that encode two amino acid substitutions: one is in the membrane protein at amino acid 35 (M-35), the other in non-structural (NS) protein 4B at NS4B-95. These common substitutions may be important in the process of attenuation of viscerotropic disease for humans and monkeys, and/or may be involved in loss of mosquito competence of the vaccine viruses.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acids / analysis
  • Animals
  • Chlorocebus aethiops
  • DNA, Viral / analysis
  • France
  • Genes, Viral*
  • Genome, Viral*
  • Haplorhini
  • Mice
  • Molecular Sequence Data
  • Nucleotides / analysis
  • Vaccines, Attenuated / genetics
  • Vero Cells
  • Viral Envelope Proteins / chemistry
  • Viral Envelope Proteins / genetics
  • Viral Structural Proteins / genetics*
  • Viral Vaccines / genetics*
  • Viral Vaccines / isolation & purification
  • Yellow fever virus / genetics*
  • Yellow fever virus / immunology*
  • Yellow fever virus / isolation & purification

Substances

  • Amino Acids
  • DNA, Viral
  • Nucleotides
  • Vaccines, Attenuated
  • Viral Envelope Proteins
  • Viral Structural Proteins
  • Viral Vaccines
  • yellow fever virus envelope protein E

Associated data

  • GENBANK/U21055
  • GENBANK/U21056