Characterization of the Safener-Induced Glutathione S-transferase Isoform II From Maize

Planta. 1995;196(2):295-302. doi: 10.1007/BF00201388.

Abstract

The safener-induced maize (Zea mays L.) glutathione S-transferase, GST II (EC 2.5.1.18) and another predominant isoform, GST I, were purified from extracts of maize roots treated with the safeners R-25788 (N,N-diallyl-2-dichloroacetamide) or R-29148 (3-dichloroacetyl-2,2,5-trimethyl-1,3-oxazolidone). The isoforms GST I and GST II are respectively a homodimer of 29-kDa (GST-29) subunits and a heterodimer of 29- and 27-kDa (GST-27) subunits, while GST I is twice as active with 1-chloro-2,4-dinitrobenzene as GST II, GST II is about seven times more active against the herbicide, alachlor. Western blotting using antisera raised against GST-29 and GST-27 showed that GST-29 is present throughout the maize plant prior to safener treatment. In contrast, GST-27 is only present in roots of untreated plants but is induced in all the major aerial organs of maize after root-drenching with safener. The amino-acid sequences of proteolytic fragments of GST-27 show that it is related to GST-29 and identical to the 27-kDa subunit of GST IV.

Publication types

  • Comparative Study

MeSH terms

  • Acetamides / pharmacology*
  • Amino Acid Sequence
  • Chromatography, Affinity
  • Chromatography, Ion Exchange
  • Enzyme Induction
  • Glutathione Transferase / biosynthesis*
  • Glutathione Transferase / chemistry
  • Glutathione Transferase / isolation & purification
  • Herbicides / pharmacology*
  • Isoenzymes / biosynthesis*
  • Isoenzymes / chemistry
  • Isoenzymes / isolation & purification
  • Macromolecular Substances
  • Molecular Sequence Data
  • Oxazoles / pharmacology*
  • Sequence Homology, Amino Acid
  • Zea mays / enzymology*

Substances

  • Acetamides
  • Herbicides
  • Isoenzymes
  • Macromolecular Substances
  • Oxazoles
  • 3-(dichloroacetyl)-2,2,5-trimethyl-1,3-oxazolidine
  • dichlormid
  • Glutathione Transferase