Constitutively Expressed Oct-2 Prevents Immunoglobulin Gene Silencing in Myeloma X T Cell Hybrids

Immunity. 1994 Nov;1(8):623-34. doi: 10.1016/1074-7613(94)90034-5.

Abstract

Recent experiments involving disruption of the Oct-2 gene have shown that this largely B cell-restricted transcription factor is not required in the early stages of B cell development. However, B cells that lack Oct-2 may be blocked from differentiation past the surface immunoglobulin-positive stage. To identify a possible function for Oct-2 in the late stage immunoglobulin-secreting cell, we have used the method of somatic cell fusion. When the immunoglobulin-producing myeloma MPC11 is fused to a T lymphoma, Oct-2 production ceases, as does the expression of immunoglobulin, J chain, and several other B cell-specific gene products. In the present study, we show that by preventing the loss of Oct-2 in the hybrid cells, we can preserve expression of all other tested B cell-specific genes. These results establish a central role for Oct-2 in maintaining the genetic program of the immunoglobulin-secreting plasmacyte.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • B-Lymphocytes / physiology
  • Base Sequence
  • Cell Line
  • DNA / analysis
  • DNA-Binding Proteins / physiology
  • Gene Expression Regulation*
  • Genes, Immunoglobulin*
  • Humans
  • Hybrid Cells
  • Immunoglobulin J-Chains / biosynthesis
  • Immunoglobulin gamma-Chains / biosynthesis
  • Immunoglobulin gamma-Chains / genetics
  • Immunoglobulin kappa-Chains / biosynthesis
  • Immunoglobulin kappa-Chains / genetics
  • Lymphoma, T-Cell
  • Mice
  • Models, Genetic
  • Molecular Sequence Data
  • Multiple Myeloma
  • Nuclear Proteins / metabolism
  • Octamer Transcription Factor-2
  • RNA, Messenger / analysis
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Transcription Factors / physiology*
  • Transfection

Substances

  • DNA-Binding Proteins
  • Immunoglobulin J-Chains
  • Immunoglobulin gamma-Chains
  • Immunoglobulin kappa-Chains
  • Nuclear Proteins
  • Octamer Transcription Factor-2
  • POU2F2 protein, human
  • Pou2f2 protein, mouse
  • RNA, Messenger
  • Transcription Factors
  • DNA