On the cellular basis of immunological T cell memory

Immunity. 1995 Jan;2(1):37-43. doi: 10.1016/1074-7613(95)90077-2.


We have studied memory in T cell receptor (TCR) transgenic mice expressing a Db-restricted TCR specific for the male peptide (H-Y). CD8+ T cells from female TCR transgenic C57BL/6 (B6) mice were activated by transferring them into X-irradiated male (B6 x bm12)F1 hybrid recipients. Subsequently, they were highly purified by cell sorting and transferred for various lengths of time into female B6 nu/nu recipient mice. Other nu/nu recipient mice received highly purified naive T cells expressing the transgenic TCR. The functional potential of naive and "memory" T cells was analyzed by stimulation with male cells in vivo. The results show that memory cells can be derived from activated T cells and persist in the absence of antigen for at least 13 weeks. Naive and memory T cells differ in that memory T cells give a more vigorous and sustained response than naive T cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD8-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / transplantation
  • Female
  • H-2 Antigens / immunology*
  • H-Y Antigen / immunology*
  • Histocompatibility Antigen H-2D
  • Immunization
  • Immunologic Memory / physiology*
  • Immunotherapy, Adoptive
  • Lymphocyte Activation
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Nude
  • Mice, Transgenic
  • Radiation Chimera
  • Receptors, Antigen, T-Cell / genetics
  • Receptors, Antigen, T-Cell / immunology*


  • H-2 Antigens
  • H-Y Antigen
  • Histocompatibility Antigen H-2D
  • Receptors, Antigen, T-Cell