A Nonmammalian Homolog of the PAF1 Gene (Zellweger Syndrome) Discovered as a Gene Involved in Caryogamy in the Fungus Podospora Anserina

Cell. 1995 Jun 30;81(7):1043-51. doi: 10.1016/s0092-8674(05)80009-1.

Abstract

The car1 gene of the filamentous fungus Podospora anserina was cloned by complementation of a mutant defective for caryogamy (nuclear fusion), a process required for sexual sporulation. This gene encodes a protein that shows similarity to the mammalian PAF1 protein (Zellweger syndrome). Besides sequence similarity, the two proteins share a transmembrane domain and the same type of zinc finger motif. A combination of molecular, physiological, genetical, and ultrastructural approaches gave evidence that the P. anserina car1 protein is actually a peroxisomal protein. This study shows that peroxisomes are required at a specific stage of sexual development, at least in P. anserina, and that a functional homolog of the PAF1 gene is present in a lower eucaryote.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arginase / biosynthesis
  • Arginase / genetics*
  • Cloning, Molecular
  • Cosmids
  • Fungal Proteins / biosynthesis
  • Fungal Proteins / genetics*
  • Genes, Fungal*
  • Humans
  • Membrane Proteins / biosynthesis
  • Membrane Proteins / genetics*
  • Microbodies / metabolism
  • Microbodies / ultrastructure*
  • Microscopy, Electron
  • Molecular Sequence Data
  • Peroxisomal Biogenesis Factor 2
  • Plasmids
  • Recombinant Proteins / biosynthesis
  • Sequence Deletion
  • Sequence Homology, Amino Acid
  • Xylariales / genetics*
  • Xylariales / growth & development
  • Xylariales / ultrastructure
  • Zellweger Syndrome / genetics*

Substances

  • Fungal Proteins
  • Membrane Proteins
  • Recombinant Proteins
  • Peroxisomal Biogenesis Factor 2
  • Arginase

Associated data

  • GENBANK/X87329