EMR1, an unusual member in the family of hormone receptors with seven transmembrane segments

Genomics. 1995 Mar 20;26(2):334-44. doi: 10.1016/0888-7543(95)80218-b.

Abstract

Proteins with seven transmembrane segments (7TM) define a superfamily of receptors (7TM receptors) sharing the same topology: an extracellular N-terminus, three extramembranous loops on either side of the plasma membrane, and a cytoplasmic C-terminal tail. Upon ligand binding, cytoplasmic portions of the activated receptor interact with heterotrimeric G-coupled proteins to induce various second messengers. A small group, recently recognized on the basis of homologous primary amino acid sequences, comprises receptors to hormones of the secretin/vasoactive intestinal peptide/glucagon family, parathyroid hormone and parathyroid hormone-related peptides, growth hormone-releasing factor, corticotropin-releasing factor, and calcitonin. A cDNA, extracted from a neuroectodermal cDNA library, was predicted to encode a new 886-amino-acid protein with three distinct domains. The C-terminal third contains the seven hydrophobic segments and characteristic residues that allow the protein to be readily aligned with the various hormone receptors in the family. Six egf-like modules, at the N-terminus of the predicted mature protein, are separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Because of its unique characteristics, this putative egf module-containing, mucin-like hormone receptor has been named EMR1. Southern analysis of a panel of somatic cell hybrids and fluorescence in situ hybridization have assigned the EMR1 gene to human chromosome 19p13.3.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Calcium-Binding Proteins
  • Chromosomes, Human, Pair 19
  • Consensus Sequence
  • Cricetinae
  • DNA, Complementary / genetics
  • Epidermal Growth Factor / chemistry
  • Genes*
  • Humans
  • Hybrid Cells
  • Membrane Glycoproteins / chemistry
  • Membrane Glycoproteins / genetics*
  • Molecular Sequence Data
  • Mucins / chemistry
  • Mucins / genetics*
  • Multigene Family*
  • Protein Conformation
  • RNA, Heterogeneous Nuclear / genetics
  • Receptors, Cell Surface / chemistry
  • Receptors, G-Protein-Coupled
  • Sequence Alignment
  • Sequence Homology, Amino Acid

Substances

  • Calcium-Binding Proteins
  • DNA, Complementary
  • EMR1 protein, human
  • Membrane Glycoproteins
  • Mucins
  • RNA, Heterogeneous Nuclear
  • Receptors, Cell Surface
  • Receptors, G-Protein-Coupled
  • Epidermal Growth Factor

Associated data

  • GENBANK/X81479