Cloning and Mapping of the Mouse Alpha 7-neuronal Nicotinic Acetylcholine Receptor

Genomics. 1995 Mar 20;26(2):399-402. doi: 10.1016/0888-7543(95)80228-e.

Abstract

We report the isolation of cDNA clones for the mouse alpha 7 neuronal nicotinic acetylcholine receptor subunit (gene symbol Acra7), the only nicotinic receptor subunit known to bind alpha-bungarotoxin in mammalian brain. This gene may have relevance to nicotine sensitivity and to some electrophysiologic findings in schizophrenia. The mouse alpha 7 subunit gene encodes a protein of 502 amino acids with substantial identity to the rat (99.6%), human (92.8%), and chicken (87.5%) amino acid sequences. The alpha 7 gene was mapped to mouse chromosome 7 near the p locus with the following gene order from proximal to distal: Myod1-3.5 +/- 1.7 cM-Gas2-0.9 cM +/- 0.9 cM-D7Mit70-1.8 +/- 1.2 cM-Acra7-4.4 +/- 1.0 cM-Hras1-ps1/Igf1r/Snrp2a. The human gene was confirmed to map to the homologous region of human chromosome 15q13-q14.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chickens / genetics
  • Chromosome Mapping*
  • Consensus Sequence
  • Crosses, Genetic
  • DNA, Complementary / genetics
  • Genes*
  • Humans
  • Mice / genetics*
  • Mice, Inbred C3H
  • Molecular Sequence Data
  • Muridae / genetics
  • Rats / genetics
  • Receptors, Nicotinic / genetics*
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Species Specificity

Substances

  • DNA, Complementary
  • Receptors, Nicotinic

Associated data

  • GENBANK/L37663