A fungal actin-related protein involved in nuclear migration

Mol Gen Genet. 1995 Jun 10;247(5):583-90. doi: 10.1007/BF00290350.


The ro-4 mutant of the filamentous fungus Neurospora crassa forms distinctive colonies in which hyphae grow into rope-like aggregates. This unusual morphology coincides with a defect in hyphal nuclear migration. The ro-4 gene was cloned from a cosmid library by complementation of the closely linked pab-2 gene. The deduced 380 amino acid protein is most similar to the vertebrate actin-related protein/centractin. The RO4 protein is not essential for cell viability, and new strains created by inducing point mutations at the ro-4 locus have a phenotype which is very similar to that of the original mutant. This study provides genetic evidence that an actin-related protein plays a role in nuclear motility. Since nuclear motility is believed to be a microtubule-dependent process, the ro-4 gene product may function as a component of the dynactin complex which activates force generation by cytoplasmic dynein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / genetics*
  • Actins / metabolism
  • Amino Acid Sequence
  • Base Sequence
  • Cell Nucleus / physiology
  • Cloning, Molecular
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Molecular Sequence Data
  • Mutation
  • Neurospora crassa / physiology


  • Actins
  • Fungal Proteins

Associated data

  • GENBANK/U14008