GTBP, a 160-kilodalton protein essential for mismatch-binding activity in human cells

Science. 1995 Jun 30;268(5219):1912-4. doi: 10.1126/science.7604265.

Abstract

DNA mismatch recognition and binding in human cells has been thought to be mediated by the hMSH2 protein. Here it is shown that the mismatch-binding factor consists of two distinct proteins, the 100-kilodalton hMSH2 and a 160-kilodalton polypeptide, GTBP (for G/T binding protein). Sequence analysis identified GTBP as a new member of the MutS homolog family. Both proteins are required for mismatch-specific binding, a result consistent with the finding that tumor-derived cell lines devoid of either protein are also devoid of mismatch-binding activity.

MeSH terms

  • Amino Acid Sequence
  • Base Composition
  • Base Sequence
  • Cloning, Molecular
  • Colorectal Neoplasms
  • DNA Repair* / genetics
  • DNA, Neoplasm / metabolism*
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • HeLa Cells
  • Humans
  • Molecular Sequence Data
  • Molecular Weight
  • Nucleic Acid Heteroduplexes / metabolism*
  • Sequence Analysis
  • Tumor Cells, Cultured

Substances

  • DNA, Neoplasm
  • DNA-Binding Proteins
  • G-T mismatch-binding protein
  • Nucleic Acid Heteroduplexes

Associated data

  • GENBANK/U28946