The TBP-TFIIA interaction in the response to acidic activators in vivo

Science. 1995 Jul 7;269(5220):75-8. doi: 10.1126/science.7604282.

Abstract

A yeast TBP mutant (N2-1) is described here that is defective specifically in responding to acidic activators in vivo. N2-1 does not support activation by Gal4, Ace1, and Gcn4, but appears unaffected for constitutive transcription, repression by the Cyc8-Tup1 and Not complexes, and transcription by polymerase I (Pol) and Pol III. In vitro, N2-1 fails to interact with TFIIA, but it associates normally with a TATA element, an acidic activation domain, and TFIIB. Fusion of the small subunit of TFIIA to N2-1 restores activation function in vivo. Thus, an efficient interaction between TBP and TFIIA is required for transcriptional activation in vivo.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • DNA-Binding Proteins / physiology
  • DNA-Directed RNA Polymerases / metabolism
  • Fungal Proteins / physiology
  • Hydrogen-Ion Concentration
  • Immediate-Early Proteins / physiology
  • Molecular Sequence Data
  • Mutation
  • Nuclear Proteins*
  • Nuclear Receptor Subfamily 4, Group A, Member 2
  • Protein Kinases / physiology
  • Repressor Proteins*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae Proteins*
  • TATA Box*
  • TATA-Box Binding Protein
  • Trans-Activators / physiology*
  • Transcription Factor TFIIA
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcription Factors / physiology
  • Transcriptional Activation*

Substances

  • CUP2 protein, S cerevisiae
  • CYC8 protein, S cerevisiae
  • DNA-Binding Proteins
  • Fungal Proteins
  • GAL4 protein, S cerevisiae
  • Immediate-Early Proteins
  • Nuclear Proteins
  • Nuclear Receptor Subfamily 4, Group A, Member 2
  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • TATA-Box Binding Protein
  • TUP1 protein, S cerevisiae
  • Trans-Activators
  • Transcription Factor TFIIA
  • Transcription Factors
  • Protein Kinases
  • DNA-Directed RNA Polymerases