Isolation and characterization of a SCT1 gene which can suppress a choline-transport mutant of Saccharomyces cerevisiae

J Biochem. 1995 Feb;117(2):447-51. doi: 10.1093/jb/117.2.447.

Abstract

The yeast Saccharomyces cerevisiae possesses a choline-transport system encoded by the CTR1 gene. We isolated a yeast gene, SCT1, that can suppress a CTR1 defect when introduced as a multicopy. The SCT1 coding frame is capable of encoding 759 residues with a calculated molecular weight of 85,734. On Northern blot analysis, an RNA species that hybridized with the coding region was detected in the total RNA of the wild-type yeast. The transcription of SCT1 is constitutive. The primary translation product contains three membrane-spanning domains, a PEST-like sequence, and a glutamic acid-rich sequence at the C terminal end. Gene disruption experiments showed that SCT1 is not an essential gene under the standard culture conditions. SCT1 did not suppress a null mutant of ctr1, indicating that a mutant form of choline transporter is necessary for the suppression caused by SCT1.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Biological Transport
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Choline / metabolism*
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Fungal Proteins / genetics
  • Genes, Fungal*
  • Genotype
  • Membrane Transport Proteins*
  • Molecular Sequence Data
  • Mutation*
  • Protein Biosynthesis
  • Restriction Mapping
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / metabolism*
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism

Substances

  • Carrier Proteins
  • DNA-Binding Proteins
  • Fungal Proteins
  • Membrane Transport Proteins
  • Transcription Factors
  • choline transporter
  • Choline

Associated data

  • GENBANK/D38256