Phase-contrast radiographs of nonstained rat cerebellar specimen

Med Phys. 1995 Apr;22(4):375-9. doi: 10.1118/1.597472.

Abstract

Phase-contrast radiography using an x-ray interferometer is presented for observing organic matter. High sensitivity of phase-contrast radiography is demonstrated with a rat cerebellar specimen without staining it with a contrast medium. The layer structure of the cerebellum can be observed in the obtained image while there is no clear structure in the corresponding absorption-contrast image. Quantitative image analysis is made possible by converting an x-ray interference pattern to an x-ray phase shift distribution. The lipid distribution in the cerebellum is discussed by evaluating x-ray phase shifts before and after lipid removal.

MeSH terms

  • Animals
  • Cerebellum / cytology*
  • Cerebellum / diagnostic imaging*
  • In Vitro Techniques
  • Interferometry
  • Mathematics
  • Radiography / instrumentation
  • Radiography / methods*
  • Rats
  • Sensitivity and Specificity