Platelet-activating factor stimulates gastric acid secretion in isolated rabbit gastric glands

Am J Physiol. 1995 Jun;268(6 Pt 1):G889-94. doi: 10.1152/ajpgi.1995.268.6.G889.


We examined the effect of platelet-activating factor (PAF) on gastric acid secretion by isolated rabbit gastric glands as determined by [14C]aminopyrine ([14C]AP) uptake. PAF, histamine, and carbachol time- and concentration-dependently stimulated [14C]AP uptake, with estimated half-maximal effective concentrations of 60 pM, 0.25 microM, and 0.1 microM, respectively. PAF-induced [14C]AP uptake was inhibited by the specific PAF antagonists BN-50727 and SR-27417 and by the proton pump inhibitors omeprazole and lansoprazole. However, the H2-receptor antagonist famotidine had no effect. Buffering extracellular Ca2+ by ethylene glycol-bis(beta-amino-ethyl ether)-N,N,N',N'-tetraacetic acid resulted in a shift to the right of the time-course effect of PAF without altering the maximal response, whereas buffering intracellular Ca2+ by 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid 2-acetoxymethyl ester, as well as blocking Ca2+ channels by verapamil, inhibited PAF-induced [14C]AP uptake. Intracellular Ca2+ concentration in isolated rabbit gastric glands, as measured by fura 2-acetoxymethyl ester, concentration-dependently increased in response to PAF, to a maximum of 1.5-fold for 0.1 microM. These results suggest that PAF stimulates gastric acid secretion via specific receptors activating intracellular Ca2+ mobilization, which could be located on the parietal cells.

MeSH terms

  • Aminopyrine / metabolism
  • Animals
  • Azepines / pharmacology
  • Biological Transport / drug effects
  • Calcium / metabolism
  • Carbachol / pharmacology
  • Dose-Response Relationship, Drug
  • Egtazic Acid / analogs & derivatives
  • Egtazic Acid / pharmacology
  • Famotidine / pharmacology
  • Fluorescent Dyes
  • Gastric Acid / metabolism*
  • Gastric Mucosa / drug effects*
  • Gastric Mucosa / metabolism
  • Histamine / pharmacology
  • In Vitro Techniques
  • Kinetics
  • Male
  • Platelet Activating Factor / antagonists & inhibitors
  • Platelet Activating Factor / pharmacology*
  • Rabbits
  • Thiazoles / pharmacology
  • Thienopyridines
  • Triazoles / pharmacology
  • Verapamil / pharmacology


  • Azepines
  • Fluorescent Dyes
  • Platelet Activating Factor
  • Thiazoles
  • Thienopyridines
  • Triazoles
  • Aminopyrine
  • Egtazic Acid
  • Famotidine
  • Histamine
  • Carbachol
  • Verapamil
  • 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid
  • Calcium
  • Setipafant
  • Foropafant