Conversion of D-arabinose to D-erythroascorbic acid and oxalic acid in Sclerotinia sclerotiorum

Biochem Biophys Res Commun. 1995 Jul 6;212(1):196-203. doi: 10.1006/bbrc.1995.1956.


D-glycero-Pent-2-enono-1,4-lactone (trivial name: D-erythroascorbic acid) occurs in the phytopathogen, Sclerotinia sclerotiorum (Lib.) de Bary, where it has a potential role as precursor of oxalic acid. On Glc/yeast/malt medium, S. sclerotiorum produces only nominal amounts of D-erythroascorbic acid but even partial replacement of Glc by D-Ara increases production of erythroascorbic acid and oxalic acid. Use of D-[1-14C]-, -[3-14C]-, or -[6-14C]Glc and D-[5-3H]-, -[2-14C,5-3H]-, or -[UL-14C]Ara provide additional information on erythroascorbic acid biosynthesis and cleavage. The latter process resembles that obtained by peroxygenation of erythroascorbic acid in alkaline solution. An unknown erythroascorbic acid-like compound also occurs in both Glc- and Ara-based cultures.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Arabinose / metabolism*
  • Ascomycota / growth & development
  • Ascomycota / metabolism*
  • Ascorbic Acid / isolation & purification
  • Ascorbic Acid / metabolism*
  • Biotransformation
  • Carbon Radioisotopes
  • Chromatography, High Pressure Liquid
  • Glucose / metabolism
  • Oxalates / isolation & purification
  • Oxalates / metabolism*
  • Oxalic Acid
  • Radioisotope Dilution Technique
  • Tritium


  • Carbon Radioisotopes
  • Oxalates
  • Tritium
  • isoascorbic acid
  • Oxalic Acid
  • Arabinose
  • Glucose
  • Ascorbic Acid