Cloning of ATAC, an activation-induced, chemokine-related molecule exclusively expressed in CD8+ T lymphocytes

Eur J Immunol. 1995 Jun;25(6):1744-8. doi: 10.1002/eji.1830250638.


A cDNA clone, designated ATAC, was isolated from a collection of human T cell activation genes. Analysis of tissue distribution determined that ATAC mRNA of approximately 0.9 kb is exclusively expressed in activated CD8+ T cells. Induction of the ATAC gene requires stimulation by both phorbol 12-myristate 13-acetate and Ca2+ ionophore A23187 ("two-signal gene") and is fully abrogated by the immunosuppressive agent cyclosporin A. Upon stimulation, ATAC mRNA is detectable within 30 min, maximal expression is seen after 4 h. The polypeptide encoded by the open reading frame of ATAC mRNA is 114 amino acids long with a calculated M(r) of 12.52 kDa. The structural features predict the cleavage and secretion of a mature ATAC protein of approximately 10 kDa from the 12.52-kDa precursor. ATAC is highly similar to a very recently identified murine molecule designated lymphotactin both at the cDNA (73.8% identity) and the protein (61.4% identity) levels, and related to members of the C-C and C-X-C chemokine families. Two variants of the ATAC protein were expressed and tested for chemotaxis and Ca2+ release on a variety of target cells. The ATAC gene was located to chromosome 1q23.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • CD8-Positive T-Lymphocytes / metabolism*
  • Chromosome Banding
  • Chromosome Mapping
  • Chromosomes, Human, Pair 1 / genetics
  • Cloning, Molecular
  • Cytokines / biosynthesis
  • Cytokines / genetics*
  • DNA, Complementary / isolation & purification
  • Humans
  • Lymphocyte Activation / genetics
  • Molecular Sequence Data


  • Cytokines
  • DNA, Complementary

Associated data

  • GENBANK/X86474