Simultaneous measurements of Ca2+ in the intracellular stores and the cytosol of hepatocytes during hormone-induced Ca2+ oscillations

FEBS Lett. 1995 Jul 10;368(1):165-8. doi: 10.1016/0014-5793(95)00632-j.

Abstract

Simultaneous Ca2+ measurements in the cytosol and intracellular stores (IS) of rat hepatocytes were performed using two Ca(2+)-sensitive probes (Fluo-3 and Mag-fura-2), and combined whole-cell patch clamp and fluorescence microscopy. A steady-state Ca2+ concentration of approximately 630 microM was estimated in the IS. alpha 1-Adrenergic stimulation induced periodic elevations of cytosolic Ca2+ and parallel synchronized transient declines in the IS. Subsequent application of the intracellular Ca(2+)-pump inhibitor thapsigargin resulted in a release of Ca2+ from the IS to reach a level of Ca2+ depletion much lower than the lowest transient decline observed during the oscillations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aniline Compounds
  • Animals
  • Calcium / metabolism*
  • Cytosol / metabolism
  • Fluorescent Dyes
  • Fura-2 / analogs & derivatives
  • Hormones / physiology*
  • Liver / cytology
  • Liver / metabolism*
  • Male
  • Phenylephrine / pharmacology
  • Rats
  • Rats, Wistar
  • Terpenes / pharmacology
  • Thapsigargin
  • Xanthenes

Substances

  • Aniline Compounds
  • Fluorescent Dyes
  • Hormones
  • Terpenes
  • Xanthenes
  • 2-(2-(5-carboxy)oxazole)-5-hydroxy-6-aminobenzofuran-N,N,O-triacetic acid
  • Phenylephrine
  • Fluo-3
  • Thapsigargin
  • Calcium
  • Fura-2