High frequency of allelic imbalance at chromosome region 16q22-23 in human breast cancer: correlation with high PgR and low S phase

Int J Cancer. 1995 Apr 21;64(2):112-6. doi: 10.1002/ijc.2910640207.


The loss of genetic material from a specific chromosome region in tumors suggests that presence of tumor-suppressor genes. Loss of heterozygosity (LOH) or allelic imbalance (AI) on the long arm of chromosome 16 is a known event in sporadic breast cancer. To locate the commonly deleted regions, and therefore (a) candidate tumor-suppressor gene(s), a deletion map of chromosome 16 was made, using 10 microsatellite markers on 150 sporadic breast tumors. The 3 smallest regions of overlap (SRO) were detected on the long arm of chromosome 16. Allelic imbalance was observed with at least one marker in 67% of the tumors. One marker, D16S421, at the 16q22-23 region, showed the highest allelic imbalance, 58%. Tumors with and without AI on 16q were tested for correlation with clinico-pathological features of the tumors such as estrogen- and progesterone-receptor content (ER and PgR), age at diagnosis, tumor size, node status, histological type, S-phase fraction, AI on chromosome 3p, and ploidy. A correlation was found between AI on 16q and high PgR content, also low S-phase fraction (99% confidence limits). A comparison of tumors with and without AI at the D16S421 marker locus revealed a slight correlation with high PgR content. The survival data showed no difference between patients with AI on 16q and those with a normal allele pattern on the long arm of chromosome 16.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles*
  • Base Sequence
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / metabolism*
  • Chromosome Deletion*
  • Chromosome Mapping
  • Chromosomes, Human, Pair 16*
  • DNA, Neoplasm / genetics
  • Heterozygote
  • Humans
  • Middle Aged
  • Molecular Sequence Data
  • Multivariate Analysis
  • Polymerase Chain Reaction
  • Proportional Hazards Models
  • Receptors, Progesterone / analysis*
  • S Phase*
  • Survival Analysis


  • DNA, Neoplasm
  • Receptors, Progesterone