Cloning and expression of mitochondrial translational elongation factor Ts from bovine and human liver

J Biol Chem. 1995 Jul 21;270(29):17243-9. doi: 10.1074/jbc.270.29.17243.

Abstract

The sequences of the cDNAs for the mitochondrial translational elongation factor Ts (EF-Tsmt) from bovine and human liver have been obtained. The deduced amino acid sequence of bovine liver EF-Tsmt is 338 residues in length and includes a 55-amino acid signal peptide and a mature protein of 283 residues. The sequence of the mature form of bovine EF-Tsmt is 91% identical to that of human EF-Tsmt and 29% identical to Escherichia coli EF-Ts. Southern analysis indicates that there are two genes for EF-Tsmt in bovine liver chromosomal DNA. A 224-base pair intron is located near the 5'-end of at least one of these genes. Northern analysis using a human multiple tissue blot indicates that EF-Tsmt is expressed in all tissues, with the highest levels of expression in skeletal muscle, liver, and kidney. Both the mature and precursor forms of bovine liver EF-Tsmt have been expressed in E. coli as histidine-tagged proteins. The mature form of EF-Tsmt forms a complex with E. coli elongation factor Tu. This complex is active in poly(U)-directed polymerization of phenylalanine. The precursor form is expressed as a 42-kDa protein, which is rapidly degraded in the cell.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cattle
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Humans
  • Mitochondria, Liver / chemistry*
  • Molecular Sequence Data
  • Peptide Elongation Factors / biosynthesis
  • Peptide Elongation Factors / chemistry
  • Peptide Elongation Factors / genetics*
  • RNA, Messenger / analysis
  • Recombinant Proteins / biosynthesis

Substances

  • Peptide Elongation Factors
  • RNA, Messenger
  • Recombinant Proteins
  • elongation factor Ts

Associated data

  • GENBANK/L37935
  • GENBANK/L37936