Abstract
A rapid and simple method of detecting enteropathogenic Escherichia coli (EPEC) was developed. The procedure is based on amplifying by the PCR method a 326-bp region of the bundle-forming pilus gene of EPEC. The oligonucleotide DNA primers used in this procedure did not amplify DNA of any other bacterial enteropathogens tested. The procedure was 100% specific for EPEC strains that exhibit a characteristic pattern of attachment (localized adherence) to HeLa cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteriological Techniques / statistics & numerical data
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Base Sequence
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DNA Primers / genetics
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DNA, Bacterial / genetics
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Diarrhea / microbiology
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Escherichia coli / classification
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Escherichia coli / genetics*
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Escherichia coli / pathogenicity*
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Escherichia coli Infections / microbiology
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Evaluation Studies as Topic
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Fimbriae, Bacterial
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Genes, Bacterial*
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Humans
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Infant
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Molecular Sequence Data
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Polymerase Chain Reaction / methods*
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Polymerase Chain Reaction / statistics & numerical data
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Sensitivity and Specificity
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Serotyping
Substances
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DNA Primers
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DNA, Bacterial