Identification of enteropathogenic Escherichia coli by PCR-based detection of the bundle-forming pilus gene

J Clin Microbiol. 1995 May;33(5):1375-7. doi: 10.1128/jcm.33.5.1375-1377.1995.

Abstract

A rapid and simple method of detecting enteropathogenic Escherichia coli (EPEC) was developed. The procedure is based on amplifying by the PCR method a 326-bp region of the bundle-forming pilus gene of EPEC. The oligonucleotide DNA primers used in this procedure did not amplify DNA of any other bacterial enteropathogens tested. The procedure was 100% specific for EPEC strains that exhibit a characteristic pattern of attachment (localized adherence) to HeLa cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriological Techniques / statistics & numerical data
  • Base Sequence
  • DNA Primers / genetics
  • DNA, Bacterial / genetics
  • Diarrhea / microbiology
  • Escherichia coli / classification
  • Escherichia coli / genetics*
  • Escherichia coli / pathogenicity*
  • Escherichia coli Infections / microbiology
  • Evaluation Studies as Topic
  • Fimbriae, Bacterial
  • Genes, Bacterial*
  • Humans
  • Infant
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Polymerase Chain Reaction / statistics & numerical data
  • Sensitivity and Specificity
  • Serotyping

Substances

  • DNA Primers
  • DNA, Bacterial