State of the art: typing Mycobacterium tuberculosis

J Hosp Infect. 1995 Mar;29(3):169-76. doi: 10.1016/0195-6701(95)90326-7.

Abstract

After years of decline, infections due to Mycobacterium tuberculosis appear to be increasing in the developed world. This has led to a search for improved methods of typing strains in order to answer new questions concerning the epidemiology of tuberculosis. Owing to the phenotypic and genotypic homogeneity of the species, classical fingerprinting methods, including phage typing, serotyping and multilocus enzyme electrophoresis, have proven ineffective. However, new methods based on the analysis of repetitive DNA sequences have been introduced and are now being used to elucidate the emerging pattern of disease.

Publication types

  • Review

MeSH terms

  • Bacterial Typing Techniques* / trends
  • Bacteriophage Typing / methods
  • DNA Fingerprinting
  • DNA, Bacterial / genetics
  • Humans
  • Mycobacterium tuberculosis / classification*
  • Mycobacterium tuberculosis / genetics
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Repetitive Sequences, Nucleic Acid / genetics
  • Tuberculosis / microbiology

Substances

  • DNA, Bacterial