Targeted disruption of mouse EGF receptor: effect of genetic background on mutant phenotype

Science. 1995 Jul 14;269(5221):230-4. doi: 10.1126/science.7618084.


Gene targeting was used to create a null allele at the epidermal growth factor receptor locus (Egfr). The phenotype was dependent on genetic background. EGFR deficiency on a CF-1 background resulted in peri-implantation death due to degeneration of the inner cell mass. On a 129/Sv background, homozygous mutants died at mid-gestation due to placental defects; on a CD-1 background, the mutants lived for up to 3 weeks and showed abnormalities in skin, kidney, brain, liver, and gastrointestinal tract. The multiple abnormalities associated with EGFR deficiency indicate that the receptor is involved in a wide range of cellular activities.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Abnormalities, Multiple / genetics*
  • Animals
  • Base Sequence
  • Brain / abnormalities
  • Brain / cytology
  • Cell Division
  • Digestive System / cytology
  • Digestive System Abnormalities
  • Embryonic and Fetal Development*
  • ErbB Receptors / deficiency
  • ErbB Receptors / genetics*
  • ErbB Receptors / physiology*
  • Female
  • Gene Targeting*
  • Hair / abnormalities
  • Homozygote
  • Kidney / cytology
  • Lung / cytology
  • Male
  • Mice
  • Molecular Sequence Data
  • Mutation
  • Phenotype
  • Skin / cytology
  • Skin Abnormalities


  • ErbB Receptors