DNA fingerprinting by pulsed-field gel electrophoresis to investigate a nosocomial pneumonia caused by Legionella bozemanii serogroup 1

Appl Environ Microbiol. 1995 Jul;61(7):2759-61. doi: 10.1128/aem.61.7.2759-2761.1995.


We typed 18 isolates of Legionella bozemanii obtained from clinical and environmental sources by pulsed-field gel electrophoresis. Each of the unrelated strains showed individual restriction patterns of the genomic DNA when either the SfiI or NotI restriction enzyme was used. One strain isolated from a patient with nosocomial legionellosis and two strains from the corresponding hospital water supply were indistinguishable, arguing for a transmission of L. bozemanii from the water supply to the patient. In conclusion, macrorestriction analysis is a valuable tool for studies of the molecular epidemiology of L. bozemanii.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cross Infection / microbiology*
  • DNA Fingerprinting*
  • DNA, Bacterial / analysis*
  • Electrophoresis, Gel, Pulsed-Field
  • Legionella / genetics
  • Legionella / isolation & purification*
  • Pneumonia, Bacterial / microbiology*


  • DNA, Bacterial