Characterization of three types of human alpha s1-casein mRNA transcripts

Biochem J. 1995 Jul 1;309 ( Pt 1)(Pt 1):237-42. doi: 10.1042/bj3090237.


Here we report the molecular cloning and sequencing of three types of human alpha s1-casein transcripts and present evidence indicating that exon skipping is responsible for deleted mRNA transcripts. The largest transcript comprised 981 bp encoding a signal peptide of 15 amino acids followed by the mature alpha s1-casein sequence of 170 amino acids. Human alpha s1-casein has been reported to exist naturally as a multimer in complex with kappa-casein in mature human milk, thereby being unique among alpha s1-caseins [Rasmussen, Due and Petersen (1995) Comp. Biochem. Physiol., in the press]. The present demonstration of three cysteines in the mature protein provides a molecular explanation of the interactions in this complex. Tissue-specific expression of human alpha s1-casein was indicated by Northern-blot analysis. In addition, two cryptic exons were localized in the bovine alpha s1-casein gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing
  • Amino Acid Sequence
  • Base Sequence
  • Caseins / genetics*
  • Caseins / metabolism
  • Cloning, Molecular
  • DNA, Complementary
  • Exons
  • Humans
  • Molecular Sequence Data
  • Phosphorylation
  • RNA, Messenger / genetics*
  • Sequence Homology, Amino Acid


  • Caseins
  • DNA, Complementary
  • RNA, Messenger

Associated data

  • GENBANK/X78416