Cloning and analysis of a Cryptosporidium parvum gene encoding a protein with homology to cytoplasmic form Hsp70

J Eukaryot Microbiol. Jul-Aug 1995;42(4):416-22. doi: 10.1111/j.1550-7408.1995.tb01605.x.

Abstract

An intronless gene encoding a protein of 674 amino acid residues with a molecular mass of 73,403 Da showing homology to the cytoplasmic form of the 70 kDa heat shock proteins has been cloned and sequenced from the intestinal pathogen Cryptosporidium parvum. Monospecific polyclonal antibodies obtained to recombinant protein recognized a single band with an approximate molecular mass of 70 kDa on a Western blot of C. parvum proteins, as well as the 70 kDa heat shock protein from bovine brain. Southern blot analysis suggested the gene was single copy in the C. parvum genome. Eleven perfect repeats of the sequence GGMP were found in the predicted protein near the carboxyl terminus.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal
  • Base Sequence
  • Blotting, Southern
  • Blotting, Western
  • Brain / metabolism
  • Cattle
  • Cloning, Molecular / methods
  • Cryptosporidium parvum / genetics*
  • DNA Primers
  • Drosophila melanogaster / genetics
  • Genes, Protozoan*
  • Genomic Library
  • HSP70 Heat-Shock Proteins / genetics*
  • Molecular Sequence Data
  • Molecular Weight
  • Polymerase Chain Reaction / methods
  • Protozoan Proteins / genetics*
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid

Substances

  • Antibodies, Monoclonal
  • DNA Primers
  • HSP70 Heat-Shock Proteins
  • Protozoan Proteins

Associated data

  • GENBANK/U11761