PCR-mediated recombination and mutagenesis. SOEing together tailor-made genes

Mol Biotechnol. 1995 Apr;3(2):93-9. doi: 10.1007/BF02789105.


Gene Splicing by Overlap Extension (gene SOEing) is a sequence-independent method for site-directed mutagenesis and/or recombination of DNA molecules. It is based on the idea that a PCR product can be engineered by adding or changing sequences at its ends so that the product can itself be used to prime DNA synthesis in a subsequent overlap-extension reaction to create mutant or recombinant molecules. As the engineered genes are created in vitro without reliance on host organisms or restriction sites, gene SOEing provides a powerful and versatile tool for genetic investigation and engineering.

MeSH terms

  • Base Sequence
  • DNA Primers / chemistry
  • DNA Primers / genetics
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed*
  • Polymerase Chain Reaction / methods*
  • Protein Engineering
  • Recombinant Proteins / genetics*
  • Recombination, Genetic*


  • DNA Primers
  • Recombinant Proteins