Structural organization of the gene encoding the human iron-sulfur subunit of succinate dehydrogenase

Gene. 1995 Jul 4;159(2):249-53. doi: 10.1016/0378-1119(95)00162-y.


The iron-sulfur protein (Ip) subunit of succinate dehydrogenase (SDH and complex II) of the respiratory chain is highly conserved in evolution [Gould et al., Proc. Natl. Acad. Sci. USA 86 (1989) 1934-1938]. We have cloned the entire human Ip cDNA, as well as the Ip-encoding gene (SDH-B) from two genomic human libraries. The cDNA contains a coding sequence of 840 nt, flanked by a 5'-UTR of 133 nt and a 3'-UTR of 123 nt. The entire transcript is encoded by eight exons within approx. 40 kb. The seven introns range in size from 0.75 kb to > 11 kb, and they appear to be of the 'late' intron class. Approx. 5 kb of upstream sequence was also cloned, and approx. 2.4 kb of the promoter region were sequenced and analyzed for consensus elements binding potential transcription factors and transcriptional activators.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Exons / genetics
  • Genomic Library
  • Humans
  • Introns / genetics
  • Iron-Sulfur Proteins / genetics*
  • Mitochondria / enzymology*
  • Molecular Sequence Data
  • Promoter Regions, Genetic / genetics
  • Protein Conformation
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Succinate Dehydrogenase / genetics*


  • DNA, Complementary
  • Iron-Sulfur Proteins
  • Succinate Dehydrogenase

Associated data

  • GENBANK/U17248
  • GENBANK/U17296
  • GENBANK/U17880
  • GENBANK/U17881
  • GENBANK/U17882
  • GENBANK/U17883
  • GENBANK/U17884
  • GENBANK/U17885
  • GENBANK/U17886