EGFR gene amplification--rearrangement in human glioblastomas

Int J Cancer. 1995 Jul 17;62(2):145-8. doi: 10.1002/ijc.2910620206.


Immunostaining using an affinity-purified rabbit polyclonal antibody against the extracellular domain of the epidermal-growth-factor receptor (EGFR) showed over-expression occurring in a fraction of tumor cells in 17 out of 18 human glioblastomas and in a majority of cells in 7 of the 18. Southern-blotting technique using a full-length EGFR cDNA probe showed a variable degree of amplification in 10 of the 17 glioblastomas, which was associated with EGFR over-expression in each case. In 2 of the glioblastomas with EGFR gene amplification, a rearrangement of the gene affecting the extracellular domain of the receptor was identified and DNA sequence analyses revealed an identical deletion-rearrangement of 801 base pairs between exons 2 to 7, resulting in an in-frame fusion of exons 1 and 8.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Brain Neoplasms / genetics*
  • Cell Line
  • DNA Primers / chemistry
  • DNA, Neoplasm / genetics
  • ErbB Receptors / genetics*
  • Gene Amplification*
  • Gene Expression Regulation, Neoplastic
  • Gene Rearrangement*
  • Glioblastoma / genetics*
  • Humans
  • Molecular Sequence Data
  • RNA Splicing
  • RNA, Neoplasm / genetics


  • DNA Primers
  • DNA, Neoplasm
  • RNA, Neoplasm
  • ErbB Receptors