Monoclonal antibody AT8 recognises tau protein phosphorylated at both serine 202 and threonine 205

Neurosci Lett. 1995 Apr 21;189(3):167-9. doi: 10.1016/0304-3940(95)11484-e.


Hyperphosphorylated microtubule-associated protein tau is the major component of the paired helical filament of Alzheimer's disease. Phosphorylation-dependent anti-tau antibodies are being used to identify specific amino acids that are phosphorylated in tau from normal brain and Alzheimer's disease brain. As such, monoclonal antibody AT8 is widely used. By a combination of site-directed mutagenesis of recombinant tau and in vitro phosphorylation, we show that AT8 requires tau protein to be phosphorylated at both serine 202 and threonine 205 (using the numbering of the longest human brain tau isoform.

MeSH terms

  • Amino Acid Sequence
  • Antibodies, Monoclonal
  • Cloning, Molecular
  • DNA, Complementary / metabolism
  • Escherichia coli / metabolism
  • Humans
  • Molecular Sequence Data
  • Phosphorylation
  • Protein Kinases / metabolism
  • Recombinant Proteins / metabolism
  • Serine / metabolism*
  • Threonine / metabolism*
  • tau Proteins / metabolism*


  • Antibodies, Monoclonal
  • DNA, Complementary
  • Recombinant Proteins
  • tau Proteins
  • Threonine
  • Serine
  • Protein Kinases