Cloning of rat KATP-2 channel and decreased expression in pancreatic islets of male Zucker diabetic fatty rats

Biochem Biophys Res Commun. 1995 Jul 26;212(3):894-9. doi: 10.1006/bbrc.1995.2053.


ATP-regulated potassium channels play a key role in regulating insulin secretion. We have isolated cDNA clones from a RINm5F insulinoma cell cDNA library that encode a protein, KATP-2, whose sequence shows 72% identity with the rat heart potassium channel KATP. RNA blotting showed that KATP-2 mRNA was present at high levels in brain and undetectable in heart, spleen, lung, liver, skeletal muscle, kidney and testis. A quantitative RT-PCR assay indicated that there were 1.85 +/- 0.32 x 10(5) molecules of KATP-2 mRNA per microgram of total RNA in pancreatic islets from nondiabetic rats. The levels of KATP-2 mRNA were reduced by 34% in islets from diabetic Zucker diabetic fatty male rats, a model of non-insulin dependent diabetes mellitus, compared to their lean nondiabetic littermates (p < 0.05), suggesting that decreased expression of KATP-2 may contribute to beta-cell dysfunction in this animal model.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphate / metabolism*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA Primers / genetics
  • Diabetes Mellitus, Experimental / genetics
  • Diabetes Mellitus, Experimental / metabolism
  • Islets of Langerhans / metabolism*
  • Male
  • Molecular Sequence Data
  • Obesity / genetics
  • Obesity / metabolism
  • Polymerase Chain Reaction
  • Potassium Channels / genetics*
  • Potassium Channels / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Zucker
  • Sequence Homology, Amino Acid
  • Tissue Distribution


  • DNA Primers
  • Potassium Channels
  • RNA, Messenger
  • Adenosine Triphosphate

Associated data

  • GENBANK/U21087