Plasma 8-epi-PGF2 Alpha Levels Are Elevated in Individuals With Non-Insulin Dependent Diabetes Mellitus

FEBS Lett. 1995 Jul 17;368(2):225-9. doi: 10.1016/0014-5793(95)00649-t.

Abstract

This study reports plasma levels of a specific nonenzymatic peroxidation product of arachidonic acid, esterified 8-epi-PGF2 alpha, from healthy- and NIDDM individuals as an index of oxidative stress in vivo. Plasma 8-epi-PGF2 alpha was isolated by solid-phase extraction on a C18 followed by an NH2 cartridge and analyzed by GC-MS/NICI as PFB-ester/TMS-ether derivative. We found that the average concentration of esterified 8-epi-PGF2 alpha among NIDDM subjects (0.93 +/- 0.07 nM, n = 39) was higher (P < 0.0001, Mann-Whitney test) than in healthy individuals (0.28 +/- 0.04 nM, n = 15). These data indicate that NIDDM is associated with increased plasma lipid peroxidation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aspirin / pharmacology
  • Chromatography, Gas / methods
  • Diabetes Mellitus, Type 2 / blood*
  • Dinoprost / analogs & derivatives*
  • Dinoprost / biosynthesis
  • Dinoprost / blood
  • Esterification
  • Humans
  • Indomethacin / pharmacology
  • Mass Spectrometry / methods
  • Models, Chemical
  • Oxidative Stress

Substances

  • 8-epi-prostaglandin F2alpha
  • Dinoprost
  • Aspirin
  • Indomethacin