Diffuse vesicular distribution of Rab3D in the polarized neuroendocrine cell line AtT-20

FEBS Lett. 1995 Jul 17;368(2):271-5. doi: 10.1016/0014-5793(95)00671-u.


The neuroendocrine cell line AtT-20 has two types of storage vesicles: dense core granules and synaptic vesicles, both sequestered at the tip of the processes. Here we show that Rab3D protein, which is abundant in fat cells, is also expressed in AtT-20 cells. Differently from Rab3A, which is localized in secretory vesicles accumulated at the tips, Rab3D has a diffuse vesicular distribution in the cytoplasm of the cell body, the processes and the tips. In AtT-20 cells, Rab3D may define a regulated secretory pathway which functions independently from cell polarity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Line
  • Cell Membrane / chemistry*
  • Cytoplasm / chemistry*
  • GTP-Binding Proteins / analysis*
  • Mice
  • Molecular Sequence Data
  • Nerve Tissue Proteins / analysis*
  • Neurosecretory Systems / chemistry*
  • Neurosecretory Systems / cytology
  • rab3 GTP-Binding Proteins


  • Nerve Tissue Proteins
  • GTP-Binding Proteins
  • rab3 GTP-Binding Proteins